Fig. 5. Effect of palmitate and/or unsaturated fatty acids on the abundance of proteins implicated in the regulation of mitochondrial function. L6 myotubes were incubated for 16 h in media containing D-glucose (5 mM) supplemented with or without palmitate (PA, 0.4 mM) or unsaturated fatty acids palmitoleate (PO, C16:1), Oleate (OL, C18:1) or linoleate (LO, C18:2) at 0.4 mM as indicated prior to cell lysis and (A) immunoblot and (B) quantitative analysis of PGC1α, SDHA, COX4.1, ANT1, TFAM, NRF1, NRF2, UCP3, Catalase, and MnSOD2. Data in (B) are presented as mean ± SEM from three or four separate experiments (one sample/condition/experiment). The asterisk indicates a significant change (P<0.05) to the control condition (lacking any fatty acid additions) or between the indicated bars.